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A simultaneous on-line solid-phase extraction (SPE) and liquid chromatography-mass spectrometry (LC-MS) method has been established for the simultaneous determination of twelve phenolic acids, gallic acid, hydroxybenzoic acid, vanillic acid, syringic acid, 4-caffeoylquinic acid, ferulic acid, rutin, mongolic acid, protocatechuic acid, salicylic acid, p-coumaric acid and sinapic acid, in plant samples. The on-line SPE and LC-MS method was developed and validated for the determination of phenolic acids in plant samples. Phenolic acids were enriched by a C18 cartridge, and the organic compounds in the cartridge were then washed off with 5mL acetonitrile. The washed organic phases were collected in glass vials for LC-MS analysis. Phenolic acids were separated on a Zorbax C18 column using a gradient mobile phase of acetonitrile and water containing 0.1% formic acid. Phenolic acids were detected by electrospray ionization-tandem mass spectrometry in the positive-ion mode. The method was applied to the determination of phenolic acids in sample types such as grass powder, stone fruits, seeds and others. The results indicated that the method is suitable for the simultaneous determination of phenolic acids in plant samples.FONT
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