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The blood was diluted in tubes containing various amounts of different near-infrared light (NIR) sensitizers and the coagulation profiles were measured at various exposure times. Both sensitizers examined–bathophenanthroline disulfonate (BPDS) and imidazole redox dyes (IR)-produce reactive singlet oxygen, which leads to hemoglobin lysis. The preformed thrombin-antithrombin III complex (TAT III) was used as a procoagulant reagent. Both IR and BPDS were shown to produce a time-dependent increase in TAT III concentration. The coagulation reaction, however, was relatively slow for BPDS, which may be attributed to the formation of large aggregates of blue-shifted BPDS-Hb-Fe(II) complexes. The monomeric IR dye produced a much faster rate of coagulation, which may be attributed to the reduced aggregation of this dye. The standard clotting time, activated partial thromboplastin time (APTT), and thrombin time (TT) gave correspondingly different profiles. The most significant finding was that the addition of BPDS, relative to IR, prolonged the TT by over 30% when the exposure time was 0.5 s. The observed prolongation of the TT is likely to be due to adsorption of BPDS-Hb complexes to the thrombocyte surface and is consistent with a previously postulated model in which the TAT III concentration profile in the blood is a function of the concentration and adsorption of the reaction components./****************************************************************************
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